IIBIO   27936
INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cell Culture Evaluation Of Epo Neuroprotection And Neuroplasticity
Autor/es:
BURGI, MARIA DE MILAGROS; CAMILA SCORTICATI; APARICIO GABRIELA; OGGERO, MARCOS; KRATJE, RICARDO
Lugar:
copenaghem
Reunión:
Congreso; Esact 2019; 2019
Resumen:
Background and novelty: Neurodegenerative diseases affect the nervous system with an incidence increasing every year. In thissense, human erythropoietin (EPO) has a leading role because its antiapoptotic, antiinflammatory, antioxidant and antiproliferativeeffects have been observed in neural tissues. Thus, the recombinant form of EPO (rEPO) might be a potential tool for neuroprotectionand neurogenesis of brain-damaged areas.Experimental approach: The in vitro neuroprotective activity of rEPO was determined by measuring the capacity of the cytokineto reverse the staurosporine (STP)-induced apoptosis using a murine neuroblastoma cell line (N2a) and primary cultures fromhippocampal neurons (HNPC). Neuroplasticity was determined by measuring neuritogenesis in N2a cells and filopodia and synapsesformation in HNPC.Results and discussion: The neuroprotection studies demonstrated that rEPO was capable to significantly reverse the STP-inducedapoptosis in a dose-response way, both in N2a cells as in HNPC (between p