INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Investigating the role of microRNAs during choroid plexus /ependyma differentiation
PAULA GONZALEZ; JUAN PABLO FEDEDA; PAULA ANABELLA AGUIRRE
Congreso; Congress of the Latin American SOciety for developmental Biology; 2019
The choroid plexus (CP)/ependyma are essential tissues that maintain the brain homeostasis by generating the cerebrospinal fluid and promoting its circulation, providing signaling space and anatomical support throughout development. Despite the important function of these multiciliated epitheliums (MEs), little is known about how their differentiation is regulated.Using in situ hybridizations, we found that several members of miR-34/449, an essential vertebrate microRNA family, are highly expressed in the CP. By using qRT-PCR, we found that miR-34/449 family member miR449a is expressed throughout CP development, from E13.5 to P30.miR-34/449 is known to play important roles in the regulation of multiciliated cells in the respiratory tract and the oviduct. In order to investigate a possible role of miR-34/449 on CP?s ME differentiation, we studied the expression of different CP markers in a miR-34/449 KO mouse model. Using qRT-PCR, we found that the expression of the apical membrane marker AQP1 was completely de-regulated in adult CPs of miR-34/449 KO mice, suggesting a role in the differentiation of the ME. Moreover, the expression of LHX2, a transcription factor whose inhibition is necessary for the early specification of CP, was also abnormal in miR-34/449 KO mice.Altogether, these results are consistent with an important role of miR-34/449 on CP development. Further studies are necessary to dissect the regulatory functions of the miR-34/449 family during CP differentiation.