The Chagas Antigen and Epitope Atlas: deep serological surveys of human Chagas Disease populations

BRACCO L; NOLAN M; KESPER JR N; MARCIPAR I; SALAS-SARDUY, EMIR; TORRICO F; VILLAR JC; AGÜERO F; RICCI A; RAMSEY J; ALTCHEH J; MARCO JD

Granada

Congreso; BSP Trypanosomiasis & Leishmaniasis Seminar 2020; 2020

British Society of Parasitology

During an infection, the immune system produces antibodies against pathogens. With time, the immune repertoires of infected individuals become specific to the history of infections and thus represent a rich source of diagnostic markers. Until now, a complete description of antibody specificities in different individuals has been hindered by the lack of powerful tools. Here, using high-density peptide arrays we examined the global human antibody repertoire developed by Chagas Disease patients. Arrays displaying 2.8 million unique peptides from T. cruzi strains CL-Brener (TcVI) and Sylvio X10 (TcI) were assayed with serum samples from infected subjects from Argentina, Bolivia, Brazil, Colombia, Mexico and the US, and negative and Leishmaniasis samples. In the presentation we will describe this collection of antigens and epitopes. This dataset will enable serological studies of human antibody repertoires in Chagas Disease at an unprecedented depth and granularity.