IIBIO   27936
INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Joint processing of apoptotic cells and Pseudomonas aeruginosa by macrophages
Autor/es:
MV PEPE; PA ARIAS; A KIERBEL; AV JAGER ; V TRIBULATTI
Lugar:
Parana
Reunión:
Congreso; LIV Reunion Anual de la SAIB; 2018
Institución organizadora:
SAIB
Resumen:
<!-- /* Font Definitions */@font-face{font-family:Cambria;panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-parent:"";margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;margin-left:0cm;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Times New Roman";mso-ascii-font-family:Cambria;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Cambria;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Cambria;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;}@page Section1{size:612.0pt 792.0pt;margin:72.0pt 90.0pt 72.0pt 90.0pt;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.Section1{page:Section1;}--> Pseudomonas aeruginosa (PA) is an opportunisticpathogen that infects vulnerable patients such as those with cystic fibrosis(CF). CF is characterized by an increased number of apoptotic cells in theairways as well as impaired efferocytosis: a process in which apoptotic cells areengulf and dispose of by other cells. We have shownthat PA binds to apoptotic cells and is internalized by epithelial cellsthrough efferocytosis. Bacteria are then eliminated intracellularly. Herewe demonstrate that bacteria-laden apoptotic cells are targets for J774macrophage-like cells and Bone Marrow-Derived Macrophages (BMM). Macrophagesinternalize apoptotic cells and PA individually or together, constituting threedifferentiated cell populations: macrophages with apoptotic cells, with PA, and with both. Classically or alternatively activated macrophagesdisplay enhanced phagocytic or efferocytic programs, respectively. Our aim isto evaluate the response elicited by macrophages facing both stimuli (i.e.bacteria and apoptotic cells) simultaneously. To study bacterialclearance we measured intracellular survival over time. Both types ofmacrophage cells eliminate PA, which was found insideLAMP1-positive vesicles suggesting that its final fate is elimination insidelysosomes. Also apoptotic material was found inside these vesicles. We alsoanalyzed the phagocytic and efferocytic efficiency. The presence of PA diminishesapoptotic cell uptake. However, the presence of apoptotic cells does not affectthe uptake/elimination of bacteria, suggesting that macrophages displayedrather a phagocytic phenotype non-withstanding the presence of apoptotic cells.