IIBIO   27936
INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
GALECTIN-8 ENHANCES T CELL RESPONSE BY PROMOTION OF ANTIGEN INTERNALIZATION AND PROCESSING
Autor/es:
CAMPETELLA, OSCAR; CARABELLI, JULIETA; CARABELLI, JULIETA; PRATO, CECILIA ARAHÍ; TRIBULATTI, MARÍA VIRGINIA; PRATO, CECILIA ARAHÍ; TRIBULATTI, MARÍA VIRGINIA; CAMPETELLA, OSCAR
Revista:
iScience
Editorial:
Cell Press
Referencias:
Año: 2020
ISSN:
2589-0042
Resumen:
Galectin-8 (Gal-8) is a mammalian lectin endowed with immunostimulatory ability. In the present work, we demonstrate that Gal-8-glycan interactions on the surface of antigen-presenting cells (APCs) promote antigen binding and internalization, independently from antigen nature. Both Gal-8 and antigen were together internalized andlocalized in early endosomes. Interestingly, antigen processing by APCs was alsoaccelerated in the presence of Gal-8 as a separate mechanism, distinct from the increasedantigen internalization. Moreover, APCs pulsed together with antigen and Gal-8 were ableto activate cognate CD4+ T cells more efficiently than those pulsed with antigen alone. Thisenhanced antigen presentation was still evident in the absence of costimulatory signalsand APCs-derived soluble mediators. Therefore, our results provide evidence for as yetunrecognized mechanism by which Gal-8 stimulates the elicitation of the immune response in a lectin-dependent manner, by inducing antigen uptake and processing upon lattice formation at APCs surface.