IIBIO   27936
INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
New human isolates of Trypanosoma cruzi confirm the predominance of hybrid lineages in domestic transmission cycles of the Argentinean Chaco.
Autor/es:
MACCHIAVERNA NP; BALOUZ V; MACCHIAVERNA NP; BALOUZ V; ENRIQUEZ GF; GÜRTLER RE; ENRIQUEZ GF; GÜRTLER RE; BUSCAGLIA C; CARDINAL MV; BUSCAGLIA C; CARDINAL MV
Revista:
Infection, Genetics and Evolution
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2018 vol. 66 p. 229 - 235
ISSN:
1567-1348
Resumen:
Trypanosoma cruzi, the etiological agent of Chagas disease, was initially classified into 6 Discrete Typing Units(DTUs). The hybrid DTUs TcV and TcVI are the most frequent in domestic transmission cycles throughout theSouthern Cone countries of South America. Here, we genotyped parasite isolates from human residents in Pampadel Indio municipality, Chaco, to further characterize the structure of T. cruzi populations, and to assess thedegree of overlapping between the domestic and sylvatic transmission cycles. Artificial xenodiagnostic tests wereperformed to blood samples from 125 T. cruzi-seropositive people (age range, 3?70 years) who represented14.3% of all seropositive residents identified. Parasites were obtained from feces of T. cruzi-infected Triatomainfestans examined 30 or 60 days after blood-feeding, and grown in vitro. The cultured parasites were genotypedby means of two PCR-based protocols. DTUs were determined from 39 (31%) patients residing in 28 dwellings.The only DTUs identified were TcV (92%) and TcVI (8?36%). Households with more than one parasite isolateconsistently displayed the same DTU. Further sequencing of a fragment of the TcMK gene from selected samplesargue against the occurrence of mixed TcV-TcVI infections in the study population. Sequencing data revealed anunexpected degree of genetic variability within TcV including two apparently robust subgroups of isolates. Ourresults for human residents confirm the predominance of hybrid lineages (TcV and to a much lesser extent TcVI)and the absence of sylvatic genotypes (TcI and TcIII) in (peri)domestic transmission cycles in the ArgentineanChaco area.