INTECH   27907
INSTITUTO TECNOLOGICO DE CHASCOMUS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
TRANSCRIPTOMIC ANALYSIS REVEALS THE ACTION MECHANISM OF SIRODESMIN PL TOXIN IN BRASSICA NAPUS
Autor/es:
ROSLI H.; RUIZ O.A.; POMBO K.; ROMERO F.M.; IDNURM A.; CANDACE E.; GARRIZ A.; ROSSI F.R.
Lugar:
MENDOZA
Reunión:
Congreso; SAIB-SAMIGE 2020; 2020
Institución organizadora:
SAIB-SAMIGE
Resumen:
BRASSICA NAPUS (colza, oilseed rape) is currently the second-largest produced oilseed crop worldwide. Like most crops, B. napus is continuously exposed to a myriad of microorganisms. PHOMA STEM CANKER (also known as the Blackleg disease) is caused by a complex of LEPTOSPHAERIA species (L. maculans and L. biglobosa), and is one of the most important diseases of B. napus and other Brassica species. This disease results in important economic losses each season, as it reduces yield by restricting water and nutrient transport through the stem, resulting in premature senescence. It has been demonstrated that some plant pathogenic fungi can produce secondary metabolite toxins as part of a multifaceted strategy to increase its infection and virulence in plants. Sirodesmin PL is the major component of the phytotoxic extracts produced by L. maculans. This toxin is a member of the epipolythiodioxopiperazine (EPTs) class of fungal secondary metabolites, which are characterized by a sulphur-bridged dioxopiperazine ring. The disulphide bridge allows ETPs to cross-link proteins via cysteine residues, and to generate reactive oxygen species through redox cycling. It has been demonstrated that L. maculans mutants unable to produce Sirodesmin PL are less virulent on stems of B. napus. Previously, we showed that Sirodesmin PL requires functional thiols for its toxicity and that it modifies the redox status in B. napus cotyledons. Nevertheless, the toxicity mechanisms of this toxin are yet far from being understood. Here, we present results from a global transcriptomic study after the treatment of B. napus cotyledons with Sirodesmin PL. Changes in the transcriptome were analyzed at 16 and 48 hours post-treatment. In this way, the amount of up-regulated differentially expressed genes (DEGs) were larger than those downregulated at both times post-treatment. To identify the biological processes, molecular functions and cellular components that are affected by Sirodesmin PL treatment, we performed Gene Ontology (GO) term enrichment on upregulated and downregulated DEG sets. Biological process related to cellular nitrogen compound metabolism, amine metabolism, oxidation-reduction process, sulphur compound metabolism, and transmembrane transport were upregulated. On the other hand, biological processes related to photosynthesis, chlorophyll biosynthesis, carbohydrate metabolism, and oxidation-reduction process were downregulated. Finally, several genes involved in the biosynthesis and signaling of plant defense hormones, including jasmonic acid and ethylene, were upregulated after Sirodesmin PL treatment. This study makes a significant contribution to understand the molecular responses of B. napus to Sirodesmin PL for the development of effective strategies in L. maculans-resistance breeding.