INTECH   27907
INSTITUTO TECNOLOGICO DE CHASCOMUS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The central nervous system acts as a transducer of stress-induced masculinization through corticotropin-releasing hormone b.
Autor/es:
DIANA CASTAÑEDA CORTÉS; GUSTAVO M. SOMOZA; VALERIE LANGOIS; LUISA F. ARIAS PADILLA; JUAN I. FERNANDINO
Lugar:
Chascomús
Reunión:
Congreso; IV Taller de Biología Celular y del Desarrollo; 2018
Resumen:
Exposure to environmental stressors during early development has important implicationsfor rescheduling many cellularandmolecular mechanisms.In manyfish species,environmental stressors, like hightemperatures(HT), causeanincreaseincortisollevels.Inturn, thismechanisminducessex reversalof genotypicfemales, overridinggeneticfactorsrelatedto developmentof thegonad. However,theinvolvementof thebraininthisprocessisnot wellclarified.Inthepresentwork,we investigatedthemRNAlevelsof corticotropin-releasinghormoneb(crhb)anditsreceptors(crhr1andcrhr2), andfoundout that they wereup-regulatedatHT duringthecriticalperiod ofgonadalsex determinationinmedaka(Oryziaslatipes),i.e.,whenthegonadalprimordiumissexuallybipotential.In ordertoclarifytheirrolesin sex reversal, biallelicmutants forcrhr1and crhr2 wereproduced by CRISPR/Cas9technology.Remarkably,biallelicmutantofbothloci(crhr1and crhr2)didnotundergo female-to-malesexreversal uponHT exposition,whereasmutantsfor either crhr1 or crhr2 showed partial,orintersexphenotypes,suggestingthat bothcrh receptorsare requiredfor HT-induced masculinization.Inhibitionofthisprocessin double crhrsmutantscould be successfully rescued throughthe administrationofthedownstreameffectorofthehypothalamic-pituitary- interrenalaxis, the cortisol.Takentogether,theseresultsrevealedforthefirsttimetheparticipation ofthecentral nervoussystemactingasatransducerofmasculinizationinducedbythermalstress.