INSTITUTO TECNOLOGICO DE CHASCOMUS
Unidad Ejecutora - UE
congresos y reuniones científicas
Los genes AtPUT que codifican transportadores de poliaminas en Arabidopsis son regulados a nivel transcripcional por las mismas poliaminas
GUERRERO-GONZÁLEZ, ML; RODRÍGUEZ-KESSLER, M; ARENAS-HUERTERO, C; MÉNDEZ-IBERRI, AE; GONZALEZ, ME; JIMÉNEZ-BREMONT, JF
Congreso; XXXII Reunión Argentina y XVI Congreso Latinoamericano de Fisiología Vegetal; 2018
Polyamines are essential regulatory molecules in many cellular and physiological processes. The main polyamines are putrescine, spermidineand spermine. In plants, the PUT (Polyamine uptake transporter) family was recently described to be responsible for polyamine intake. In the Arabidopsis thaliana genome five genes encoding PUTs (AtPUT1-AtPUT5) have been identified. Herein we found that the AtPUTgenes were transcriptionally regulated by polyamines in a time and concentration dependent manner in Arabidopsis plantlets. In addition, theeffect of low intracellular polyamine levels on the expression of the AtPUTgenes was assessed in the amiR:ADC-L2line, which is characterizedby ca. 85% reduction in total polyamine content. Among the Arabidopsis AtPUTs, the AtPUT2 gene was selected for functional characterization.It was found that the spatio-temporal expression of the AtPUT2 gene was modulated by polyamines and other plant growth regulators, as wellas at different stages of the Arabidopsis ontogeny in the promAtPUT2::GUS reporter line. Finally, the effect of polyamines, hormones (ABA) andosmotic stress inducers (mannitol and sorbitol) on the phenotype of the put2-1T-DNA insertional line was assessed.