INVESTIGADORES
ROSSI Franco Ruben
artículos
Título:
Study of Oxidative Stress and Enotoxicity by Arsenic Contamination in Glycine max. L
Autor/es:
MARSA SILVANA; ROSSI FRANCO; VALDEZ HUGO; GOMEZ CASATI DIEGO; ZIRULNIK FANNY
Revista:
International Journal of Plant & Soil Science
Editorial:
SCIENCEDOMAIN international
Referencias:
Año: 2015 vol. 8 p. 1 - 15
ISSN:
2320-7035
Resumen:
Introduction:Volcanism is an important natural origin of Arsenic (As) contamination. Aims:Oxidative stress parameters, antioxidant defense system, the expression oftranscripts involved in defense responses and the As genotoxicity wereanalyzed. Study Design:The plants were separated into two groups (Control and As-treated ones) untilthey were exposed to As (2.5 and 5 ppm) at day 10. Methodology:Biochemical, molecular and cytogenetic determinations were carried out. Results: Arsenic treated plants show growthabnormalities. The antioxidant defenses and oxidative parameters in roots andleaves showed different tissue- specific responses. Whereas leaf tissuesdisplay an active antioxidant defense, root cells are unable to produce defensemolecules and showoxidative stress. Moreover, the analysis of antioxidant enzymes such asascorbate peroxidase and superoxide dismutase, showed higher activity in leavesthan in roots, except for isocitrate dehydrogenase. The expression of GR andMAPK decreased significantly in all the treatments during the time curve. ICDHshowed an increase in its expression with 24 hours of treatment and falls at 72hours compared to their controls. Genotoxicity assays showed a decrease ofmitotic index (MI) and the presence of altered nucleus, in samples exposed for72 hours. Conclusion: Our results suggest that the physiological,biochemical, molecular and cytogenetic behavior of the plants depends on the Asconcentration and the length of the exposure.