GWAS FOR GROWTH AND WOOD QUALITY TRAITS IN EUCALYPTUS DUNNII

PAMELA VICTORIA VILLALBA; MARTÍN NAHUEL GARCÍA; AUGUSTO J. LÓPEZ; DINO PALAZZINI; MARTÍN ALBERTO MARCÓ; H. ESTEBAN HOPP; EDUARDO PABLO CAPPA; NATALIA CRISTINA AGUIRRE; JUAN GABRIEL RIVAS; CINTIA VANESA ACUÑA; PABLO PATHAUER; JAVIER OBERSCHELP; ROCÍO CARRERAS; DARIO GRATTAPAGLIA; SUSANA NOEMÍ MARCUCCI POLTRI.; CARLA VALERIA FILIPPI; MARÍA CAROLINA MARTÍNEZ; JUAN ADOLFO LÓPEZ; LEONEL HARRAND; ESTEBAN FELIPE CISNEROS; JOSÉ RODRIGUES; NORMA BEATRIZ PANIEGO

BUENOS AIRES

Congreso; 1st Congress of Women in Bioinformatics and Data Science LA; 2020

Species of Eucalyptus are among the most widely planted forest trees in the world. In Argentina,Eucalyptus breeding is mainly carried out by INTA, with Eucalyptus dunnii as one of the mainspecies targeted. This eucalypt is highly demanded by the cellulose pulp industry, especially inregions where other widely planted species are not adapted to climate conditions.In this work, a single-trait GWAS (Genome-Wide Association Study) was carried out in E. dunniifor 13 traits relevant to the species? breeding program.A population of E. dunnii (308 individuals from 72 open-pollinated families) was genotyped with:an optimized ddRADSeq (Double-digest restriction site-associated DNA sequencing) protocol(Aguirre et al., 2019) and the EUChip60K commercial SNP microarray. The SNP matrices werefiltered for quality metrics and remaining missing data imputed. A total of 27,215 SNP weredistributed along the 11 chromosomes, covering complementary genomic regions between bothgenotyping methodologies. A low extent of linkage disequilibrium was evidenced and genetic structure by DAPC showed two subpopulations consistent with the original provenances in theircentre of origin wherefrom the population under study was derived.The GWAS was carried out under a mixed linear model, including kinship matrix, for growth andwood quality traits. Eighteen associated SNPs (p