ALFALFA: GENETIC IMPROVEMENT THROUGH CRISPR/CAS9.

GOMEZ, CRISTINA; GONZALES, MATIAS; BLUMWALD, EDUARDO; STRITZLER, MARGARITA; MASSA, GABRIELA; TAJIMA, HIROMI; SOTO, GABRIELA; BOTTERO, EMILIA; PASCUAN, CECILIA; FEINGOLD, SERGIO; AYUB, NICOLÁS

capital federal

Congreso; SAIB SAMIGE; 2020

SAIB, SAMIGE

Alfalfa (M. sativa) is the most important forage worldwide, thus numerous efforts have been made to increase its productivity and resistance to different stresses. The CRISPR system (Clustered Regularly Interspaced Short Palindromic Repeats), which is part of the natural immune system of some bacteria and archeas against virus invasion, can be adapted and used as a programmable gene editing tool. Engineered CRISPR systems consists of a guide RNA (sgRNA) and a CRISPR-associated endonuclease (Cas9). The sgRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined 20 nucleotide spacer that defines the Cas9 genomic target. Gene editing through CRISPR/Cas9 became a technological revolution and a powerful tool to modify genomes in a fast, efficient and precise way. I This mechanism has been applied efficiently in plants since 2013.