Mitogenome and Nuclear-encoded Fungicide-target Genes of Thecaphora frezii - Causal Agent of Peanut Smut

MASSA, A.; LAMB, M.; CONFORTO, C; BUTELER, M.; ARIAS, R; SCHEFFLER, B.; DUKE, M.; PAREDES J.A.; RAGO, A.M.; CAZON, I; SOBOLEV, V.; SIMPSON, S.; SOAVE J.

Fungal Genomics & Biology

Longdom Publishing SL

Lugar: Bruselas; Año: 2019 vol. 9 p. 1 - 8

2165-8056

Background: Thecaphora frezii Carranza and Lindquist causes smut disease in peanut (Arachis hypogaea L.) resulting in up to35% yield losses. Fungicides have shown ineffective in controlling the disease; whereas research on the molecular basis of thatfungicide resistance has been hindered because of the lack of genetic information about T. frezii. The goal of this work was toprovide molecular information about fungicide-target loci in T. frezii, including its mitochondrial genome (mitogenome) andcritical nuclear-encoded genes.Results: Here we report the complete annotated mitogenome of T. frezii, a 123,773 bp molecule containing the standard 14genes that form part of mitochondrial complexes I, III, IV and V, 22 transfer RNAs, small and large subunits of ribosomalRNA, DNA polymerase, ribonuclease P, GII-reverse transcriptase/maturase, nine hypothetical open-reading frames and homingendonucleases (LAGLIDADG, GIY-YIG, HEG). In addition, we report the full-length cDNA sequence of T. frezii cytochrome b(cob) and cytochrome oxidase 1 (cox1) genes; as well as partial sequences of T. frezii succinate dehydrogenase (sdhb), ergosterol biosynthesis(Erg4), cytochrome P450 (cyp51), and beta tubulin (β-tubulin) genes, which are respective targets of strobilurins, quinone oxidationinhibitors, triazoles and beta-tubulin inhibitor fungicides commonly used in the peanut crop. Translation of cob and sdhb genes inthis particular T. frezii isolate suggests potential resistance to strobilurin and carboxamide fungicides.Conclusion: The mitogenome and nuclear-encoded gene sequences presented here provide the molecular tools to research T.frezii fungicide-target loci.