UFYMA   27844
UNIDAD DE FITOPATOLOGIA Y MODELIZACION AGRICOLA
Unidad Ejecutora - UE
artículos
Título:
First report of grapevine red blotch virus infecting grapevine in Argentina
Autor/es:
DEBAT, HUMBERTO; ASURMENDI, SEBASTIAN; LUNA, FACUNDO; ZAVALLO, DIEGO; MOYANO, SABRINA; GOMEZ-TALQUENCA, SEBASTIAN
Revista:
JOURNAL OF PLANT PATHOLOGY
Editorial:
EDIZIONI ETS
Referencias:
Lugar: Pisa; Año: 2019 vol. 101 p. 1239 - 1239
ISSN:
1125-4653
Resumen:
Argentina is the fifth wine producer worldwide and 10th globalgrapevine producer, with over 2,100,000 tons of grapesharvested in 2017. So far, 12 viruses have been identified ingrapevine in Argentina (Debat et al. 2019). Grapevine redblotch virus (GRBV) is the type member of the genusGrablovirus, in the family Geminiviridae (Varsani et al.2017) and the causal agent of the red blotch disease (RBV,Yepes et al. 2018). RBV was first reported in California in theUSA and is responsible for significant reduction of berry qualityand ripening, and thus, an emerging threat to grapevinecultivation. GRBV has been described in the USA, Canada,Mexico, Korea, India and Switzerland, thus far. In order toassess the presence of GRBV in Argentina, a total of 188plants were surveyed, including wine and table cultivars,and rootstock genotypes from five regions of Mendoza andSan Juan provinces of Argentina in 2018. Total RNAs fromcambial scrapings of grapevine samples were purified usingthe Spectrum Plant RNA Miniprep Kit (Sigma-Aldrich,USA). The extracted RNA was random primed,retrotranscribed and subjected to PCR using primersGRBaV-F: GCCTTGTCAGTTTGCATTCC and GRBaV-F:CTTCCGCTGTTATCACTACC, targeting a 270 nt regionof the V1 protein encoding open reading frame (ORF) ofGRBV. One sample of Vitis vinifera cv. Flame Seedless renderedan amplification product of expected size which wascloned, bi-directionally Sanger sequenced. Nucleotide sequenceanalyses revealed that GRBV-MZ (MK575527) hada 98.3% identity with GRBV isolate CYCS45 (MF795153)from Vitis vinifera cv. Cabernet Sauvignon from Washington,USA. To confirm our findings we tested the aforementionedsample with additionally designed primers GRBaV1097F:ACGAGGAATCGTTTGAATCG and GRBaV1331R:TAAACGTATGTCCACTTGCAG for the amplification byPCR, cloning and sequencing of a 235 nt fragment of the 3?region of V1 ORF of GRBV. The sequence (MK575528)showed 99.5%identity with GRBV CYCS45, confirming thatthe detected virus corresponded to GRBV. The Argentineanisolate of GRBV was found in a plant with no obvious symptomsof viral diseases. To our knowledge, this is the first reportof GRBV in Argentina.