BODY COMPOSITION CHANGES THROUGHOUT TWO GENERATIONS OF RATS FEEDING A NORMAL CALCIUM-HIGH FAT DIETARY CONTENT.

SUAREZ C; FERREIRA MONTEIRO AG; RODRIGUEZ P; GONZALES CHAVES M; PELLEGRINI GG; ZENI SN; FRIEDMAN SM

Chile

Congreso; SLAN 2009; 2009

In the last years a substantial body of evidence has emerged in support of the interrelationship between obesity and osteoporosis. On this bases the present study investigated the effect of long-term intake of normal calcium (Ca) diets -with different fat content- on body composition, in healthy rats. At weaning (21 days), female Wistar rats (n=30) were randomly assigned one of the following 3 groups (n=10 each). G1 was sacrificed in order to get basal values (Co); G2 and G3 were fed ad libitum one of the two isocaloric diets (A; fat=7% w/w or B; fat =15 % w/w) up to day=70. Then, mothers (MA1 & MB1) were mated and pups (PA1 & PB1) were evaluated at weaning. At day=70, twenty rats from PA1 & PB1 (MA2 & MB2, respectively) were mated and pups (PA2 & PB2) were studied at weaning. Body weights (BW), body fat (% BF) and water (% BW) percentages by chemical method (AOAC) and total skeleton bone mineral content (teBMC) by DXA (Lunar DPX-L) were evaluated. Results (mean±SD): BW increased across reproductive cycles being significant in pups for group B (PB2>PB1>C0, p<0.01; MB2 vs MB1, p=0.05 and PA2, PA1, C0 p=0.05, MA2 vs MA1, p=0.05). BW increase because of the increment in %BF. Conversely, at weaning % BW (data not shown) and teBMC decrease as %BF increased (Table:different letters show significance). Conclusions: A long-term normal Ca-high fat diets leads to a progressive change in body composition with an increase in BF content and a decrease in BW and teBMC. Awarded by UBACyT O 008.   %BFP1 %BFP2 %BFM1 %BFM2 teBMC1 teBMC2 A (7%F) 7±2a 9±2a 4±1a 4±1a 38±8d 52±7d B (15%F) 12±3b 15±2c 8±5b 10±1c 41±5d 56±7d