Saliva and Serum Crosslaps Present Similar Annual and Daily Rhythm.

PELLEGRINI GG; GONZALES CHAVES MACARENA MS; SOMOSA J; FRIEDMAN SM; ZENI SN

Isla de Margarita Venezuela

Congreso; III Congreso de la región latinoamericana de la IADR; 2009

IADR

Proper management of osteoporosis involves early diagnosis, including complementary tests such as biochemical markers of bone turnover screaning. Previously we measured Carboxy terminal telopeptide of collagen type I (CTX) in human saliva finding similar response in serum and salivary samples under different conditions: normal, increased, and reduced bone remodeling. Bone resorption presents diurnal variation, with a maximum early in the morning and a nadir in the late afternoon. Seasonal change related to vitamin D status was also observed. Objetive: To determinate whether salivary bone resorption marker CTX as serum sample presents daily and annual rhythm in women. Methods: forty women (20 pre and 20 postmenopausal) from two southern cities: Comodoro Rivadavia (45-S) and Ushuaia (54-S) were studied. Blood, urine and saliva samples were collected at the end of summer and of winter according to known variations in daily rhythm. Urine and saliva were collected at 4 hours intervals during 24 hours. CTX (Biodiagnostic, Denark), 25hidroxivitamin D (Diasorin) and other determinations related to mineral metabolism were also evaluated. Results: CTX levels (ng/L) at the end of winter (1) and of summer (2) in saliva and serum were significantly higher in post. than in premenopausal women respectively. (2) salivary: 46.2±10.6 vs, 56.6±16.(1); serum: 217.8±77.1 vs. 309.6±115.6. (1) salivary: 52,5±14,3 vs 65,8±22,3 and serum: 223,1±69,2 vs. 311,5±122,9, [p<0.05]). A seasonal variation related to lower levels of 25OHD at the end of winter was observed. The 24 hours study exhibit daily rhythmicity. Conclusion: Data suggests that a simple and less invasive sample could be used to assess bone resorption replacing of serum or urine evaluations. Since dental visits are more frequent than medical check-ups, performing routine assay of salivary biomarkers of bone disease with delivering routine dental care could allow earlier diagnosis of metabolic bone diseases.