INVESTIGADORES
PELLEGRINI Gretel Gisela
congresos y reuniones científicas
Título:
Evaluation of a new bovine biomaterial to conduct bone healing
Autor/es:
PELLEGRINI GG; GONZALES CHAVES MMS; DURAN F; ORZUZA R; ZENI S
Reunión:
Congreso; American Society for Bone and Mineral Research Annual Meeting; 2017
Institución organizadora:
ASBMR
Resumen:
Pellegrini Gretel G1, 2, Gonzalez-Chaves Macarena MS1, 2, Francisco Duran2, Orzuza Ricardo1, Parodi L3, Zeni Susana N1, 21 Universidad de Buenos Aires, Facultad de Odontología, Cátedra de Bioquímica General y Bucal, Argentina.2 Instituto de Inmunología Genética y Metabolismo, Universidad de Buenos Aires-CONICET (INIGEM), Argentina.3 Comisión Nacional de Energía Atómica, División Bioterio. Centro Atómico Ezeiza, Argentina Placing biomaterials to prevent resorption and preserve the integrity of alveolar ridges after invasive dental procedures is highly recommended. Bovine bone grafts are biocompatibile and osteoconductive, allowing new bone apposition by osteoprogenitor cells. Developing new high quality biomaterials offer professionals an alternative option to obtain the most suitable solution for each individual patient. We evaluated and compared the effects of new bovine bone grafts Synergy Bone Matrix (SBM) [Odontit Implant System, Argentina] with Bio-Oss (BO) [Geistlich, Switzerland], recognized for its osteoconductive effects on the bone healing process in rabbits. We created a critical sized bone defect (CSBD) at both sides of mandibles and filled them with each biomaterial or remained unfilled (C: control). Animals were sacrificed at 4, 8 and 12 weeks. We assessed: clinical and biochemical systemic toxicity of lungs, kidney and liver pathology; bone formation and device resorption histomorphometrically; X-rays and compression and flexural biomechanical tests. None of the rabbits presented signs of systemic toxicity. C rabbits developed either fibrosis or adipose tissue independently of the time-period; new bone formation or remaining bone substitute amount did not show significant differences between SBM and BO (p=ns). CSBD in mandibles filled with both biomaterials exhibited radio-opacity indicating proper healing and gradual replacement of the bone grafts; no significant differences between SBM and BO were observed for elastic modulus (Mpa)[4 weeks: C: 13.34±1.23; BO: 47.83±4.82*; SBM: 42.59±5.21*. 8 weeks: C: 13.72±1.84; BO: 43.42±6.32*; SBM: 46.61±4.45*. 12 weeks: C: 14.61±3.1; BO: 50.57±15.7*; SBM: 49.32±18.9*]; shear modulus (Mpa) [4 weeks: C: 182.5±15.4; BO: 423.9±20.3*; SBM: 425.6±25.7*. 8 weeks: C: 185.4±12.1; BO: 397.8±30.5*; SBM: 447.4±35.6*. 12 weeks: C: 172.2±13.1; BO: 464.6±26.9*; SBM: 473.1±32.4*] and compressive strength (KgF/mm2) [4 weeks: C: 0.0595±0.0082; BO: 0.6407±0.0124*; SBM: 0.6913±0.0110*. 8 weeks: C: 0.0583±0.0098; BO: 0.6874±0.0115*; SBM: 0.6816±0.0153*. 12 weeks: C: 0.0694±0.0104; BO: 0.6983±0.0199*; SBM: 0.6956±0.178*], while C group showed significantly lower values (*p