Comparison of alpha CTX levels in healthy prepuberal children, adolescent, pre and postmenopausal women.

ZENI SN; PELLEGRINI GG; LINARI M; PIAZZA N; SOMOZA J; RIO ME

Honolulu, Hawaii, USA

Congreso; Twenty-nine Annual Meeting of the American Societt for Bone and Mineral Research.; 2007

ASBMR

Collagen type I fragments are formed by osteoclasts and same of them can be determined using an specific immunoassays. The CTX epitope EKAHDGGR comprises a DG-motif susceptible to post-translational modifications. In newly synthesised collagen this motif is in the á-CTX form, but during aging of bone it is isomerized to â-CTX form. The á-CTX form can be determined in urine using a new sandwich ELISA. The aim of the present study was to assess the ability of this marker to discriminate the degradation of new bone in different stage of aging. A total of 243 healthy subjects were included. The distribution of the subjects were: 173 prepuberal children (70 girls and 103 boys) and 30 adolescent (12 girls and 18 boys) attending public schools of Vicente Lopez, Buenos Aires and 20 premenopausal and 20 postmenopausal women). The á-CTX (Alpha CTX ELISA; Nordic Bioscience) was assessed in the second void urine. Results of á-CTX/creatinine levels (ug/mMcreatinine) as mean ±SD were:Prepuberal children: 20±7.2 (6 to<7 years); 15.7±4.5 (7 to <8 years); 14.9±6.7 ( 8 to <9 years) and 15.3±6.3 (9 to <10 years), adolescents: 4.2±1.9 (11-12 years); premenopausal women: 0.61±0.26 (36.9±7.5) and postmenopausal women: 0.93±0.58 (58.0±5.3 years). There was a significant differences among all groups however the levels of significance between postmenopausal vs. premenopausal women (p<0.05) was the lowest.The present results suggest that á-CTX marker reflects well the increase of bone resorption associated with bone modeling at childhood and with high bone turnover after menopause.