Can determination of carboxy-terminal telopeptide of type I collagen (CTX) in saliva replace determination in serum and urine?

PELLEGRINI GRETEL G; GONZALES CHAVES, G; PONCE, M.A.; FAJARDO, J; SOMOSA, JULIA; CASCO C; ZENI SUSANA N

Buenos Aires

Congreso; XXII Reunión Anual de Asociación Argentina de Osteologia y Metabolismo Mineral; 2005

Asociación Argentina de Osteologia y Metabolismo Mineral

Although oral bone loss and osteoporosis have different etiologies, they may share mechanism involved in bone mass loss. The present study was carried out to determine whether salivary and serum CTX correlate, under normal conditions and in increased bone remodeling due to estrogen deficiency. Twenty healthy women (10 pre, 10 postmenopausal) (self-reported history of medical and dental care living in Cro.Rivadavia) and adult Wistar rats (10 SHAM and 10 one week post-OVX) were studied. Fasting serum and: women total unstimulated saliva, rat stimulated saliva (pylocarpine) were collected. CTX was measured as follows: rat saliva and blood, ELISA (RatLabs, Osteometer Bio Tech), human blood, EQL (Elecsys), human saliva, ELISA (Crosslaps Osteometer Bio Tech). Results (rats: ng/10 ml, women ng/L) (mean ± SD): Saliva SHAM vs. OVX: 35.7 ± 5.6 vs. 90.0 ± 25.1 (P < 0.01), serum SHAM vs. OVX: 154.2 ± 43.5 vs. 208.8 ± 37.1(P < 0.05); saliva pre vs. postmenopausal 348.1 ± 336.3 vs. 596.1 ± 552.5 (P < 0.05), serum pre vs. postmenopausal 224.2 ± 87.5 vs. 367.5 ± 163.6 (P < 0.05). When saliva CTX was plotted against serum CTX the following correlations were obtained: (a) all studied population: r = 0.80; (b) only women: r = 0.73; (c) only rats: r = 0.62; (d) the population in estrogen depletion: r = 0.72 and (e) in estrogen deficiency: r = 0.77 (P < 0.05). Conclusions: Salivary and serum CTX correlated under normal conditions and in estrogen deficiency. Although future studies are necessary, the present results are promising as regards the potential use of a salivary-based test for CTX. Grant from Buenos Aires University M033