IVIT   27842
INSTITUTO DE VIROLOGIA E INNOVACIONES TECNOLOGICAS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The stress response evaluation in vicuñas subjected to different types of shearing
Autor/es:
MAIDANA, SS; ROMERO, S; RAMOS, NE; MIÑO, S; ODEON MM; ROMERA, SA
Lugar:
Mar del Plata
Reunión:
Congreso; VI° Reunión Científica Regional de AACyTAL y LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2019
Institución organizadora:
AACyTAL-SAIC
Resumen:
The South American camelids (SACS) ?llama (Lama glama)and vicuiia? (Vicugna vicugna) possess natural libers of exoeptional fineness. CurrenTly, vicuña ?s fiber is obtained by the practice of capture, shearing and releasing back to nature. This practice Is performed In two management models that, due to theIr status as wildlife, respond to conservation arid sustainable tise policies; captivity (INTA Abra Pampa) and Silvestre (chaku), carried out by native peoples communities.Then, the objective of this study Is to assess the level of stress in both modalitIes. The levels of glucose and protein were quantified after manual and mechanical shearing of vicuñas and defined as stress indicators. These stress indicators were assessed in ti-apping and shearIng of wild vicuñas comparatively with the shearing of vicuñas In captivity. The sample was taken in both cases immediately post shearing. The glucose concentration was measured with test strips (values expressed in mg/dl). The plasma was recovered to quantified plasma protein measurements by Lowry?s method. In addition, for standarization of interleuldnas qPCR,, primers of different interleukins were designed based on the genome of Camelus drornedarius, to assess stress at the level of gene expression. To obtain the positive controk, DNA was ectraded from tissue with a commercial kit. We evaluate the specificity of primers by end-point PCR since they are designed based on another species of camelicle deposited in the database. The results would indkate that the vicuñas mechanically sheared hoW lower conceritrations of glucose (164,Bmg/dI vs 232,6 mg/dl) and total proteins (2891.2 ug/ul vs 6287ug/ul) that manual shearing In sllvestry condition. In captivity the levels of glucose were (100,õmg/dl vs 133 mg /dl)and toti proteins (62.5ug/ulvs113 lug/ui) mechanically and manual shearing respectively. Due to the results obtained mectianical shearing generates less stress response in both sllvestry and captIvity. Develop of methodologies adapted to local needs evaluating physiological, biochemical and behavioral parameters as indicators of well-being will allow increasing the productivity ofcarneilds, redudng stress In the different managenients an added value for comniercialization of fibre, responding to the demands of an international market ethically sensitive to animal abuse and regulatory requirements for wildlife conservation and managenient.