The use of a liposome with MANalpha1-2MAN-PEG-PE molecule enhances the immunogenicity induced by a nanovaccine against BoHV-1 in the murine model and in cattle

KORNUTA CLAUDIA A; GAMMELLA, MARIELA; HECKER, YANINA; SALMASO, STEFANO; LANGELLOTTI, CECILIA; SORIA, IVANA; MOORE, DADIN PRANDO; PAPPALARDO, JUAN SEBASTIÁN; ZAMORANO, PATRICIA; BIDART, JUAN ESTEBAN; QUATTROCCHI, VALERIA; CHEUQUEPÁN VALENZUELA, FELIPE; PETROVICH TORCHILIN, VLADIMIR

Seattle

Simposio; 12th International Veterinary Immunology Symposium; 2019

American Association of Veterinary Immunologists

Bovine herpesvirus-1 (BoHV1) is an etiological agent of the bovine respiratory disease complex. In this work, BALB / c mice and cattles were used; they were immunized with a DNA vaccine that carries the secreted version of the BoHV1 glycoprotein D using as a pCIneo vector (pCIgD). A liposome containing the Manα1-2Man-PEG-PE molecule was formulated together with LPS and pCIgD (pCIgD-Man-L) was also evaluated. Groups of BALB/c mice (n = 5) were inoculated on days 0 and 20 with: pCIgD; pCIgD-MAN-L; pCIneo-MAN-L. At 20, 40 and 120dpv, the group pCIgD-MAN-L presented significant differences in the level of total antibodies against BoHV1 in contrast to the group that did not include liposome in the vaccine. When isotypes were evaluated, IgG1 levels were significantly increased (p < 0.001) in the animals that received the pCIgD-Man-L in contrast to the other groups. There was also an increase in isotypes IgG2a and IgG2b (p< 0.01) Groups of cattle (n=5) were inoculated intradermally on days 0, 30 and 60 with pCIgD; pCIgD-MAN-L; pCIneo. At 90 dpv levels of antibodies in pCIgD and pCIgD-Man-L groups were significantly higher than those in the pCIneo group. At 90dpv the animals were challenged with 1x106.81 TCID50/ml of BoHV1. Nasal swabs were obtained over 12days for virus excretion evaluation. The titers in pCIgD-Man-L were significantly lower (p< 0.01) than titers in the others groups at 5dpc. When calculating the area under the curve of excretion during the 12dpc, it was lower in pCIgD-Man-L group (4,77x106TICD50/ml in pCIneo group, 1,12x106TICD50/ml in pCIgD group and 2,62x105TICD50/ml in pCIgD-Man-L group).