An indirect ELISA validated as diagnostic and vaccines quality control tool for Bovine Parainfluenza Virus Type 3 (BPIV3)

MARIA MERCEDES ODEON; GRAZZIOTTO NOELIA; LUCIA ROCHA; MAIDANA S. S; EDDIE PISANO; VIVIANA PARREÑO; CAROLA FERRECCIO; IRENE ALVAREZ; ROMERA S.A.

Montpellier

Congreso; 18th Ecology and Evolution of Infectious Diseases meeting Montpellier - France; 2021

Serological assays, including enzyme-linked immunosorbent assays (ELISA), provide a useful tool to screen animals for the presence of antibodies (Abs) against a wide range of infectious agents (including viruses that cause respiratory disease in cattle) and are widely used in veterinary medicine to assist in the control and monitoring of disease. The aim of the present study was develop and validate of two indirect enzyme-linked immunosorbent assay (ELISA) based on semipurified bovine parainfluenza virus type 3 (BPIV3) for the detection and quantification of Abs against PI3 in serum sample from cattle and guinea pigs for both purpose diagnostic and typify/ specify the quality of vaccines. The diagnostic sensitivity and specificity of the assay was 88% and 100% for bovine samples using a threshold of corrected optical density, ODc =0.300, and 91% and 100% for guinea pig samples with a ODc =0.250.The intermediate precision of the assays expressed as the relative coefficient of variation (CV) of the positive control was 20% for bovines and 8.5% for guinea pigs. The reproducibility of both techniques obtained in inter-laboratory assays was CV=17% for bovines and 15% for guinea pigs, which met the requirements of the OIE (CV