First report of group A rotavirus and bovine coronavirus associated with neonatal calf diarrhea in the northwest of Argentina

BOK, M.; AGUIRRE, D.; PARREÑO, V.; BOK, M.; AGUIRRE, D.; PARREÑO, V.; ADURIZ, M.; SAIF, L.; MIÑO, S.; ADURIZ, M.; SAIF, L.; MIÑO, S.; BERTONI, E.; VEGA, C.; CIMINO, R. O.; BERTONI, E.; VEGA, C.; CIMINO, R. O.

TROPICAL ANIMAL HEALTH AND PRODUCTION

SPRINGER

Lugar: Berlin; Año: 2020 p. 2761 - 2768

0049-4747

Group A rotavirus (RVA) and bovine coronavirus (BCoV) are the two main viral enteropathogens associated with neonatal calfdiarrhea. The aim of the present survey was to investigate the epidemiology and the role of RVA and BCoV in the presentation ofdairy and beef calf diarrhea in Lerma Valley of Salta province, within the Northwest region of Argentina. Stool samples of calveswith or without diarrhea younger than 2 months of age were collected from 19 dairy farms and 20 beef farms between the years2014 and 2016. Stool samples were screened for RVA and BCoV detection by ELISA. Heminested multiplex RT-PCR was usedfor RVA typing and RT-PCR to confirm BCoV. Positive samples were submitted to sequencing analysis. Bovine RVA andBCoV were circulating in 63% (12/19) and 10.52% (2/19) of the dairy farms, respectively, where 9.5% (46/484) of the calveswere positives to RVA and 0.4% (2/484) to BCoV. In beef herds, RVA was detected in 40% (8/20) of the farms and in 6.75% (21/311) of the calves, without positives cases of BCoV. Molecular analysis showed that in dairy farms, G6P[11] and G10P[11] werethe prevalent RVA strains, while in beef farms, G10P[11] was the prevalent. The main finding was the detection for the first timeof a G15P[11] causing diarrhea in beef calves of Argentina that represents a new alert to be consider for future vaccine updates.Analysis of detected BCoV showed that it is related to the other circulating strains of Argentina.