IVIT   27842
INSTITUTO DE VIROLOGIA E INNOVACIONES TECNOLOGICAS
Unidad Ejecutora - UE
artículos
Título:
Development and Validation of aNovel ELISA for the Detection of Neospora caninum Antibodies in Bovine Sera
Autor/es:
GUAL I; TURCO, CECILIA; MANSILLA FC; CARDOSO NP; PEREYRA W. R.; CAPOZZO A V
Revista:
Austin J Vet Sci & Anim Husb.
Editorial:
Austin Publishing Group
Referencias:
Lugar: Texas; Año: 2019 vol. 6
ISSN:
2472-3371
Resumen:
Neospora caninum is a world-wide distributed apicomplexan parasite,causative agent of bovine neosporosis, which is one of the major causes ofreproductive losses in cattle affecting both dairy and beef industries. Severaltechniques are regularly used for the diagnosis of bovine neosporosis. Amongstthem, serological techniques are generally preferred, being IFAT the traditionallyaccepted reference method for diagnosis (gold standard). However, ELISA hasbecome the most eligible method for large-scale screening of specific antibodiesagainst N. caninum in diagnostic laboratories around the world, providing resultsthat can be easily standardized between laboratories, within a few hours at arelative low-cost. There is no local production of commercial ELISA tests nor inArgentina neither in South America, therefore they must be purchased abroad,turning unfeasible the routinely use of ELISA tests. Here we have developed andvalidated accordingly to OIE specifications a novel ELISA test based on solubleantigens form N. caninum tachyzoites to accurately determine the presence ofspecific antibodies anti- N. caninum antigens in bovine sera samples, enablingthe rapid, objective and low- cost diagnosis of bovine neosporosis in Argentina;replacing IFAT and avoiding delays and high importation taxes.