Development of an improved anesthesia protocol to increase CF1 micesurvival in a portal vein infection with Echinococcus granulosussensulato protoscoleces

DENEGRI, GUILLERMO M.; PENSEL, PATRICIA E.; SCIOSCIA, NATHALIA P.; ELISSONDO, MARÍA CELINA

Heliyon

Elsevier BV

Año: 2021 vol. 7 p. 1 - 5

2405-8440

In order to optimize the survival rate of animals, the purpose of this study was to evaluate an injectable anesthesiaprotocol for the development of a murine model of hepatic cystic echinococcosis in female CF-1 mice. Threeprotocols of injectable anesthesia were evaluated during the infection of mice with Echinococcus granulosus sensulato protoscoleces via the portal vein. The use or not of pre-anesthesia [atropine (0.4 mg/kg) and tramadol (2 mg/kg)] and the incorporation or not of yohimbine (0.5 mg/kg) (a reverser of xylazine) in mice anesthetized withketamine/xylazine 80/8 mg/kg were evaluated. Most mice treated only with ketamine/xylazine 80/8 mg/kg didnot achieve a deep surgical anesthetic plane. All mice treated with pre-anesthetic drugs achieved a deep surgicalanesthetic plane after the administration of the anesthetic cocktail. Pre-anesthetic drugs application significantlyreduced time induction of animals compared with those that received only anesthetic cocktail. Recovery time wassignificantly faster in the group that received yohimbine. Mice underwent laparotomy that did not receiveyohimbine after surgery had a survival rate of 67%, whereas in the group treated with yohimbine the survival was100 %. We recommend the protocol that applied pre-anesthetic drugs þ ketamine/xylazine 80/8 mg/kg þyohimbine, as safe and reliable for the portal vein infection of mice with protoscoleces of E. granulosus sensu lato.