Bioactive peptides of the Cry1Ab16 toxin from Bacillus thuringiensis by nanodevices films for potential biotechnological applications

PLACIDO ALEXANDRA; OLIVEIRA FARIAS EMANUEL A.; MARANI MARIELA M.; VASCONCELOS ADREANNE G.; MAFUD ANA C.; MASCARENHAS YVONNE P.; EIRAS CARLA; LEITE JOSÉ R. S. A.; DELERUE-MATOS MARÍA C.

Porto

Encuentro; 15th Iberian Peptide Meeting; 2016

Faculdade de Ciencias-Universidade do Porto

Peptides are potential candidates to meet the needs of the modern world in relation to diagnosis, disease monitoring, quality control in industry, and more recently, detection of genetically modified organisms (GMOs) and food security through the development of biosensors (Plácido et al., 2015). Cry1Ab16 is a toxin of crystalline insecticidal proteins family that has been widely used in GMOs to gain resistance to pests. Recently, many studies have focused on evaluating the potential environmental impact of this toxin, including the impact on aquatic environments. Moreover, this toxin has been detected in maternal and fetal samples during pesticide exposure studies related to genetically modified food. For the first time, in this study, peptides derived from the immunogenic Cry1Ab16 toxin (from Bacillus thuringiensis) were immobilized as layer-by-layer films (Farias et al., 2015). Given the concern about food and environmental safety, a peptide with immunogenic potential, PcL342-354C, was selected for characterization of the electrochemical. In addition, an attempt was made to optimize the electrochemical signal of the peptide through interspersion with different natural or synthetic polymers. Finally, the system was characterized using various morphological and spectroscopic techniques such as AFM, UV-Vis, XRD, and FTIR. An ITO/PEI/(PSS/PcL342-354C)n film proved to be an excellent candidate for applications in electrochemical sensors and other biotechnological applications for GMOs and environmental indicators. Acknowledgements: This work was partially supported by grants from the financial support by FAPEPI, CAPES, and CNPq. A. Plácido is gratefully to FCT by her grant SFRH/BD/97995/2013, financed by POPH?QREN?Tipologia 4.1?Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. This work has also been supported by the FCT through grant number PEst-C/EQB/LA0006/2011. ACM is indebted to FAPESP (Grant 2014/02282-6). YPM is grateful to CNPq (Grant 302674/2010-1).