INVESTIGADORES
MARANI Mariela Mirta
artículos
Título:
Thaulin-1: the first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli
Autor/es:
MARANI MARIELA M.; PEREZ LUIS O.; RODRIGUES DE ARAUJO ALYNE; PLACIDO ALEXANDRA; SOUSA CARLA; QUELEMES PATRICK A.; OLIVEIRA MAYARA; GOMES-ALVES ANA G.; PUETA MARIANA; GAMEIRO PAULA; TOMAS ANA M.; DELERUE-MATOS MARÍA C.; EATON PETER; CAMPERI SILVIA A.; BASSO NÉSTOR G.; LEITE JOSÉ R. S. A.
Revista:
GENE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2017 vol. 605 p. 70 - 80
ISSN:
0378-1119
Resumen:
Patagonia´sbiodiversity has been explored from many points of view, however, skinsecretions of native amphibians have not been evaluated for antimicrobialpeptide research until now. In this sense, Pleurodemathaul is the first amphibian specie to be studied from this large regionof South America. Analysis of cDNA-encoding peptide in skin samples allowed identification of four new antimicrobial peptides. The predicted mature peptides were synthesized and all of them showed weak or null antimicrobialactivity against Klebsiella pneumonia,Staphylococcus aureus and Escherichia coli with the exception of thaulin-1, a cationic 26-residue linear, amphipathic,Gly- and Leu-rich peptide with moderate antimicrobial activity against E. coli (MIC of 24.7 µM). AFM and SPRstudies suggested a preferential interaction between these peptides andbacterial membranes. Cytotoxicityassays showed that thaulin peptides had minimal effects at MIC concentrationstowards human and animal cells. These are the first peptides described for amphibiansof the Pleurodema genus. These findings highlight the potential of thePatagonian region?s unexplored biodiversity as a source for new moleculediscovery.