Antibacterial Activity of Novel Peptide Derived from Cry1Ab16 Toxin and Development of LbL Films for Foodborne Pathogens Control

PLACIDO ALEXANDRA; BRAGANÇA IDALINA; MARANI MARIELA M.; RODRIGUES DE ARAUJO ALYNE; VASCONCELOS ADREANNE G.; BATZIOU KRYSTALLENIA; DOMINGUES VALENTINA; EATON PETER; LEITE JOSÉ R. S. A.; DELERUE-MATOS MARÍA C.

MATERIALS SCIENCE & ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2017

0928-4931

Escherichia coli is one of the most commonetiological agents of diarrhea in developing countries. The appearance ofresistant E. coli prevents treatment theseinfections. Biotechnological applications of antimicrobial peptides can beconsidered good alternatives to treat and prevent intestinal infections by thesebacteria. The aim of this study was to evaluate the antibacterial activity of thepeptide PcL342-354C,which is derived from the toxin Cry1Ab16 from Bacillus thuringiensis, against E.coli strains. We also report the preparation, characterization andevaluation of the antibacterial activity of LbL films containing PcL342-354C. The results showed thatthe PcL342-354Cpeptide inhibited the growth of different strains of E. coli with MIC ranging from 15.62 ? 31.25 μg/mL and minimalbactericidal concentration was 250 μg/mL, indicating a potential antibacterialactivity. The morphology of an ITO/Cashew gum/PcL342-354C film was analyzed usingatomic force microscopy which showed an increase of roughness due to the increasein the number of layers. The LbL films have antibacterial activity against E. coli NCTC 9001 in both conditionstested (10 and 20 bilayers). Our results indicate that the peptide exhibits anantibacterial potential that can be tapped to develop biomaterials withantibacterial activity for use against foodborne pathogens.