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Título:
DEVELOPMENT OF DROUGHT AND SALINITY TOLERANCE BY INTRODUCTION OF A CYANOBACTERIAL FLAVODOXIN
Autor/es:
FERNÁNDEZ V; GIRÓ M; HAJIREZAEI M; GOSPARINI C; MORANDI E; CARRILLO N; ZURBRIGGEN M
Lugar:
San Miguel de Tucumán. Tucumán. Argentina
Reunión:
Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Resumen:
<!-- /* Font Definitions */ @font-face {font-family:"TimesNewRomanPSMT,Bold"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:0; mso-generic-font-family:roman; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} @font-face {font-family:"TimesNewRomanPSMT,Italic"; panose-1:0 0 0 0 0 0 0 0 0 0; mso-font-charset:0; mso-generic-font-family:roman; mso-font-format:other; mso-font-pitch:auto; mso-font-signature:3 0 0 0 1 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Drought and salinity rank first among the major factors affectingncrop yield and plant reproduction. Perturbation in water and osmotic homeostasis results in build-up of reactive oxygen species that poses a serious threat to cellular viability. Oxidative stress leads to decreased levels of ferredoxin (Fd), which is essential for electron distribution to a plethora of regulatory, dissipative and central metabolic pathways. Under these conditions, cyanobacteria and some algae induce the synthesis of the flavoprotein flavodoxin (Fld), which can provide a functional substitution of Fd in many of its crucial functions in the chloroplasts. Transgenic tobacco plants expressing Anabaena Fld in chloroplasts display enhanced tolerance to oxidative stress conditions and iron deficiency. In this work we show that in contrast to wild-type (WT) specimens these plants are also able to grow under extreme drought conditions. Physiological and biochemical data reveals a protection of the normal photosynthetic and primary metabolisms. Leaf disks of WT plants floated in NaCl solutions undergo extensive bleaching while the flavodoxin expressing counterparts maintain high levels of chlorophyll and caretonoids showing enhanced tolerance to salt-induced damage. The Fld gene-based strategy could be a useful biotechnological tool to improve crop yields and to gain at least some wastelands for agriculture