Features and applications of Ent35-MccV hybrid bacteriocin: current state and perspectives

NAVARRO, S. A.; LANZA, L.; ACUÑA, L.; BELLOMIO, A.; CHALÓN, MIRIAM C.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

SPRINGER

Año: 2020 vol. 204 p. 6067 - 6077

0175-7598

Bacteriocins are peptides of ribosomal synthesis that are active against bacteria related to the producing strain. They have beenwidely used in the food industry as biopreservatives. The generation of hybrid peptides by combining the genes that encode twodifferent bacteriocins has made it possible to study the mechanisms of action of the bacteriocins that compose them and alsodevelop new peptides with improved biotechnological applications. Hybrid bacteriocins may be obtained in several ways. In ourlaboratory, by combining enterocin CRL35 and microcin V (Ent35-MccV), we obtained a broad-spectrum peptide that is activeagainst both Gram-positive and Gram-negative bacteria. Ent35-MccVis sensitive to the action of intestinal proteases and is heatresistant, which makes it a good candidate for use as a biopreservative. For this reason, the peptide was tested in skim milk andbeef burgers as food models. We also obtained more potent variants of the hybrid by modifying the central amino acid of thehinge region that connects the two bacteriocins. This review also discusses future applications and perspectives regarding theEnt35-MccV and other hybrid peptides.