Biochemical techniques for the study of long noncoding RNAs

CHRIST, A; ARIEL FD; CRESPI, M

Paris

Conferencia; Post-transcriptional Gene Regulation in Plants; 2015

PGRP Organizing Committee

Noncoding RNAs have emerged as major components of the eukaryotic transcriptome. Genome-wide analyses revealed the existence of thousands of long noncoding RNAs (lncRNAs) in several plant species. They are involved in a wide range of regulatory mechanisms impacting on gene expression, including chromatin remodeling, modulation of alternative splicing, fine-tuning of miRNA activity, and the control of mRNA translation or accumulation. In our lab we perform alternative biochemical approaches to decipher the molecular mechanisms involving lncRNAs, such as Chromatin Immunoprecipitation (ChIP), RNA Immunoprecipitation (RIP) and Chromatin Isolation by RNA Purification (ChIRP). This variety of techniques has allowed us to determine DNA and protein partners of lncRNAs. Furthermore, Next Generation Sequencing technologies served to identify genomic regions recognized by chromatin-related lncRNAs.