Identification of a Nitric Oxide – Regulated Differentially Expressed mRNA during the lateral root development in tomato.

D'ALESSANDRO C; MUÑOZ F; ORDOÑEZ MV; RUIZ DM; SALTARINI S; SASTRE DE; GRAZIANO M; LAXALT A; LAMATINA L

Iguazu (Misiones)

Congreso; XL Reunión Anual - Sociedad Argentina de Investigación en Bioquímica y Biología Molecular (SAIB).; 2004

Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular (SAIB)

Nitric Oxide (NO) is a free radical involved in several physiological processes in plants, many of which are related with responses mediated by phytohormones. For instance, NO participates in the signaling pathway triggered by Auxins that induces the development of lateral roots and inhibits the elongation of the primary root in tomato. In this study, the technique of Differential RNA Display (DD) was used for the identification of NO-induced differentially expressed genes in tomato roots. The profile of DD showed changes in gene expression of seedlings treated with the NO donor sodium nitroprussiate (SNP). One cDNA clone (Le16) corresponding to differentially expressed transcripts was sequenced since it displayed low amounts of RNA in NO treatments in both DD and Northern Blot analysis. The nucleotide sequence of Le16 was 100% homologue with the 3’-UTR of a gene that encodes for a protein that binds to an Ethylene Reponse Element (EREBP). A DNA binding domain AP2 was found from the deduced aminoacid sequence of EREBP which is also present in many plant transcriptional regulators. These findings suggest that Le16 would act as a transcriptional regulator of genes that encode for proteins whose expression links NO and ethylene signaling cascades during the lateral root formation.