IITEMA   27339
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
In vivo biocompatibility of hydrogels based on poly-N-isopropylacrylamide (PNIPAM)
Autor/es:
BABINI, S.; RIVAROLA, CLAUDIA R.; ARRI, JIMENA; LIAUDAT, ANA C.; RODRÍGUEZ, NANCY; CAPELLA, VIRGINIA; BOSCH, PABLO
Lugar:
Virtual
Reunión:
Jornada; IV Reunión Conjunta de Sociedades de Biología de la República Argentina; 2020
Institución organizadora:
Sociedades de Biología de la República Argentina
Resumen:
Over the last decades, biomaterials have been developed with great potential to be applied as scaffolds for cell growth, expanding the alternatives toorgan and tissue transplants, within the field of tissue engineering. These scaffolds, in addition to allowing a correct in vitro development of the tissueof interest, must be accepted by the host avoiding any type of immunological rejection and must also be biostable in the time required for the desiredtissue regeneration. Instead, its use in vivo introduces a relevant variable that must be studied: the interaction of the biomaterial with the cellsbelonging to the host's immune system. Hydrogels, especially those based on poly-N-isopropylacrylamide (PNIPAM), have been among the moststudied in our group due to their similarity with the extracellular matrix (EMC). Based on this background, the aim of this study was to analyze thebiocompatibility and immunological acceptance with three-dimensional (3D) hydrogels based on PNIPAM and its co-polymer, PNIPAM-co-3%APTA (3-acrylamidopropyl chloride) trimethyl- ammonium), in murine models of the Wistar strain. For this purpose, hydrogel discs were implantedin subcutaneous pockets, after sterilizing the materials. In the controls, the same procedure was performed without implanting the hydrogel. Thehealing process was monitored for 5 days, and the materials were recovered after 3 months. After this stage, the individuals were sacrificed, andblood samples were taken for hematological and biochemical analysis. No alterations in the healing process were observed regarding to controls inboth treatments. Also, there were neither significant changes in the leukocyte formula, compatible with inflammatory processes, nor in the bloodbiochemistry that can be suggestive of kidney and liver dysfunction. Hence, these preliminary studies indicate that PNIPAM-based hydrogels do notgenerate immune rejection or significant alterations in the hematology and blood biochemistry of Wistar rats in a chronic period of 3 months.