IITEMA   27339
INSTITUTO DE INVESTIGACIONES EN TECNOLOGIAS ENERGETICAS Y MATERIALES AVANZADOS
Unidad Ejecutora - UE
artículos
Título:
Photodynamic inactivation of ESKAPE group bacterial pathogens in planktonic and biofilm cultures using metallated porphyrin-doped conjugated polymer nanoparticles
Autor/es:
MARTINEZ, SOL ROMINA; FORCONE, MARIA VIRGINIA; SPESIA, MARIANA BELÉN; PONZIO, RODRIGO ANDRES; CHESTA, CARLOS A.; IBARRA, LUIS EXEQUIEL; WENDEL, ANA BELEN; PALACIOS, RODRIGO E
Revista:
ACS Infectious Diseases
Editorial:
American Chemical Society
Referencias:
Año: 2020
ISSN:
2373-8227
Resumen:
Photodynamic inactivation (PDI) protocols using photoactive metallated porphyrin-doped conjugated polymer nanoparticles (CPNs) and blue light were developed to eliminate multidrug-resistant pathogens. CPNs-PDI protocols using varying particle concentration and irradiation doses were tested against nine pathogenic bacterial strains including antibiotic-resistant bacteria ofthe ESKAPE pathogens group. Bactericidal effect was achieved in methicillin-resistant Sthaphylococus aureus strains using low lightdoses (9.6-14.4 J/cm2); while Gram-negative bacteria required a higher light dose (28.8 J/cm2). Bacteria-CPNs interaction was studiedthrough flow cytometry taking advantage of the intrinsic CPNs fluorescence, demonstrating that CPNs efficiently bind to the bacterialenvelope. Finally, the performance of CPNs-PDI was explored in biofilms; good antibiofilm ability and almost complete eradicationwere observed for S. aureus and Escherichia coli biofilms, respectively, using confocal microscopy. Overall, we demonstrated thatCPNs-PDI is an efficient tool not only to kill superbugs as sessile cells but also to disrupt and eradicate biofilms of highly relevantpathogenic bacterial species