A natural oil increases specific anti‐OVA IgG levels and induces a cellular immune response combined with aluminum hydroxide

CAMPRA, NOELIA ANAHÍ; RAVIOLO, JOSÉ; CARIDDI, LAURA NOELIA; MONTIRONI, IVANA DALILA; MORENO, FEDERICO RUIZ; REINOSO, ELINA BEATRIZ; MALETTO, BELKYS

JOURNAL OF LEUKOCYTE BIOLOGY

FEDERATION AMER SOC EXP BIOL

Año: 2020

0741-5400

The efficacy of certain vaccines is improved by the use of adjuvants. Nowadays, the developmentof new, effective, and safe adjuvants that stimulate the innate immune response is researched. Inthis context, medicinal plants appear as a suitable alternative. Minthostachys verticillata essentialoil (EO) has demonstrated the ability to modulate mechanisms of the innate immune response.Thus, the present work aimed to evaluate the EO adjuvant effect on humoral and cellular immunity,coadministered with OVA as antigen. The chemical analysis of EO by gas chromatography?mass spectrometry revealed a predominant pulegone?menthone chemotype. EO (1.25, 2.5, or5.0 mg/ml) did not alter the viability of murine fibroblasts (3T3 cell line) neither showed signs oftoxicity in Balb/c mice inoculated subcutaneously. The serum of mice immunized with OVA + EOshowed increased levels of anti-OVA-specific antibodies of IgG1 subclass compared with the miceimmunized with OVA alone revealing an adjuvant effect of EO. The delayed type hypersensitivityshowed that the combination OVA + Al(OH)3 + EO was the best to induce a cellular immuneresponse that extended until 48 h postinjection of OVA. M. verticillata EO appears as a new, safe,and effective adjuvant,which should continue to be studied for their possible future incorporationinto vaccine formulations.