INVESTIGADORES
PERASSOLO Maria
congresos y reuniones científicas
Título:
Enhancement of anthraquinone production in Morinda citrifolia cell suspension cultures after treatment with azetidine-2-carboxylic acid and thiazolidine-4-carboxylic acid
Autor/es:
QUEVEDO, CV; PERASSOLO, M; GIULIETTI, AM; RODRÍGUEZ TALOU, JULIÁN
Lugar:
Barcelona
Reunión:
Congreso; 14th European Congress on Biotechnology.; 2009
Resumen:
Plant cell suspension cultures are attractive alternatives for large scale production of plant-derived natural products, in particular of secondary metabolites. Morinda citrifolia is a member of Rubiaceae family that produces anthraquinones (AQs), anthracene derivatives which exhibit biological interesting properties. The basal compound, anthraquinone (9, 10-dioxoanthracene), can be substituted in various ways, resulting in a great diversity of structures. Different metabolic routes are involved in AQs synthesis: shikimate pathway produces chorismic acid, which is then converted into isochorismic acid by the enzyme isochorismate synthase. The reaction of this compound with á-ketoglutaric acid generates o-succinylbenzoic acid, the precursor of A and B rings of the AQs structure. C ring is derived from an isoprene unit produced by the 2-C-methyl-D-erythritol-4-phosphate pathway. The proline cycle was proposed to be linked to the pentose phosphate pathway (PPP), since the first one generates two NADP+ which are cofactors of the two first enzymes in the PPP. The PPP produces erithrose-4-phosphate, which is substrate of the shikimate pathway. The aim of this work was to study a possible link between proline cycle and AQs production and to evaluate this link as a possible strategy for AQs accumulation. Morinda citrifolia cell suspension cultures were treated with azetidine-2-carboxylic acid (A2C; 25 and 50 µM) and thiazolidine-4-carboxylic acid (T4C; 100 and 200 µM), two proline analogs. All treatments except from A2C 25 µM showed a higher AQs content (p<0.05) compared to the control line, while only T4C 200 µM treatment increased total phenolics (TP) content after a 6-day culture. After 10 days of culture, both T4C treatments enhanced AQs and TP production compared to control. Accumulation of proline was significantly increased by all treatments after 6 days of culture, and by A2C 50 µM and both T4C treatments after 10 days of culture (p<0.01). These findings could be correlated with PPP stimulation.