INVESTIGADORES
ARRUVITO Maria Lourdes
congresos y reuniones científicas
Título:
CD32 EXPRESSION IDENTIFIES FUNCTIONALLY DISTINCT CD4+ T CELLS
Autor/es:
HOLGADO P; RAIDEN S; SANANEZ I; DE LILLO L; GONZALEZ, F; GEFFNER J; ARRUVITO L
Reunión:
Congreso; Reunión conjunta de sociedades de Biociencias 2017; 2017
Resumen:
Receptors for the Fc portion of IgG (FcyR) represent a critical link between innate and adaptive immunity. They enable IgG to trigger effector functions such as cytotoxicity and complement activation. In addition to acute disease, Respiratory Syncytial Virus (RSV) bronchiolitis is associated with asthma during infancy. Although the expression and activity of FcyRII (CD32) on neonatal CD4+ T cells could influence their function, it has not been characterized.Our aim consisted in identifying CD32 expressing T cells in cord blood mononuclear cells (CBMCs) by flow cytometry; as well as to determine whether cross-linking of FcyR by immobilized IgG modulated the pattern of secreted cytokines. Moreover, we also analyze the frequency of CD4+CD32+ T cells in RSV infected infants.Our data demonstrated that CD4+CD32+ T cells represent ~3.9% ± 0.6 of CD4+ T cells (n=10). In contrast with CD4+CD32- T cells, this subset mainly consists of naive CD45RA+ and CCR7+ cells (p