INVESTIGADORES
ARRUVITO Maria Lourdes
artículos
Título:
Respiratory Syncytial Virus (RSV) Infects CD4+ T Cells: Frequency of Circulating CD4+ RSV+ T Cells as a Marker of Disease Severity in Young Children
Autor/es:
RAIDEN, SILVINA; SANANEZ, INÉS; REMES-LENICOV, FEDERICO; PANDOLFI, JULIETA; ROMERO, CECILIA; DE LILLO, LEONARDO; CEBALLOS, ANA; GEFFNER, JORGE; ARRUVITO, LOURDES
Revista:
JOURNAL OF INFECTIOUS DISEASES
Editorial:
UNIV CHICAGO PRESS
Referencias:
Año: 2017
ISSN:
0022-1899
Resumen:
Background: Although human airway epithelial cells are the main target of respiratory syncytial virus (RSV), it also infects immune cells, such as macrophages and B cells. Whether T cells are permissive to RSV infection is unknown.Objectives: To analyze the permissiveness of CD4+ T cells to RSV infection. Methods: CD4+ and CD8+ T cells from cord blood, healthy young children and adults were challenged by RSV or co-cultured with infected HEp-2 cells. Infection, phenotype and cytokine production by T cells were analyzed by flow cytometry or ELISA. Expression of RSV antigens by circulating CD4+ T cells from infected children was analyzed by flow cytometry, and disease severity defined by standard criteria. Results: CD4+ and CD8+ T cells were productively infected by RSV. Infection decreased IL-2 and IFN- production as well as the expression of CD25 and Ki-67 by activated CD4+ T cells. RSV antigens were detected in circulating CD4+ and CD8+ T cells during severe RSV infection of young children. Interestingly, the frequency of CD4+RSV+ T cells positively correlated with disease severity. Conclusions: RSV infects CD4+ and CD8+ T cells and compromises T cell function. The frequency of circulating CD4+RSV+ T cells might represent a novel marker of severe infection.