3-D printing of biodegradable scaffolds to restore scar tissue: Design and model of a PLA proof of concept

SORDELLI, ANDREA; RINFLERCH, ADRIANA; RISK, MARCELO; VALENZUELA ALVAREZ, MATIAS; BELATTI, ANAHI; BOLONTRADE, MARCELA F.; GUZMAN, GUIDO; GUTIERREZ, LUCIANA M.; MAZZUOCOLO, LUIS

Mar del PLata

Congreso; Reunión Conjunta de Sociedades de Biociencias, LXIV Reunión Anual SAIC, Sociedad Argentina de Investigación Clínica. SAI. SAFIS; 2019

We approached the feasibility of printing biologically plausible tissue structures for organ replacement using 3D printers and PLA(polylactic acid) as biomaterial. To this end we printed tubes as scaffolds that would substitute scar tissue. By Fusion 360 we designedtubes of 14.0 mm long (tolerance of ±0.1mm) and 5.4 mm in diameter (tolerance of ±0.05mm) of 1.72 mm thick PLA, that were sterilized byUV light. The printing was done on a modified Makerparts1 printer with a 0.2mm diameter spout. Size, macrostructure and porosity wouldsuit recellularization. We are currently approaching the use of fibroblasts to adhere to the scaffold to help in the regeneration process ofthe target tissue and provide anchoring for epithelial cells. Cells from excess, discarded tissue samples (surgeries at the Dermatologyservice, HIBA) will be used to anchor on the generated structures. The choice of resorbable materials that shape suitable artificialprosthesis (scaffold) is essential, allowing the biological system to function as a bioreactor using the scaffold as the structure from whichthe new organ will be reorganized.