Bioaccumulation of carbamazepine, enalapril and sildenafil in fish under laboratory conditions

CRISTOS DIEGO; ROJO MACARENA; CARRIQUIRIBORDE PEDRO

Waco, Texas

Congreso; SETAC 9th Young Environmental Scientists Meeting (YES); 2020

Wastewater effluents are the main source of Human Pharmaceuticals (HP) in surface water bodies. Carbamazepine (CBZ), enalapril (ENA) and sildenafil (SIL) had been detected in water bodies of Argentina at concentrations ranging 0.59-26.72 µg/L and in muscle of feral fish from Río de La Plata basin with commercial interest, ranging 1.41-26.8 µg/Kg. This study aimed to assess the bioaccumulation of CBZ, ENA, and SIL in the widespread native species C. decemmaculatus exposed to concentrations 200, 500, and 100 µg/L, respectively, during 96 h with partial test media renewal every 48 h. HPs extractions were carried out by using the "Rotatory Extractor" method. Direct injection after 0.22 µm filtration was done for water chemical analyses. Analytical determinations in water and muscle were performed by HPLC-MS. The average concentration in fish corresponding to the last time of exposure for CBZ, ENA, and SIL was: 203 ± 28.9 (N=3) µg/Kg, 2.16 ± 1.86 (N=3) µg/Kg and 102 ± 7.31(N=3) µg/Kg, respectively. The empirical Bioconcentration Factors (BCF) at 96 h calculated for each one was: 0.9, 0.004 and 0.8. The uptake of HPs was evidenced and the obtained concentrations were in the order of those found in feral fish. However, for the high levels of HP assayed in this study, the empirical BCFs obtained were low, indicating no bioaccumulation.