Effects of cannabis oil on liver injure and oxdative stress in an experimental modelo of Metabolic Syndrome?.

VACCARINI C.; D'ALESSANDRO M.E.; OLIVA M.E.; SEDAN D.; VEGA JOUBERT M.B; ANDRINOLO D.

Buenos Aires

Congreso; LXVI Reunión anual de la Sociedad Argentina de Investigacion Clínica (SAIC); 2021

SAIC

Liver damage and oxidative stress are closely related to the alterations present in Metabolic Syndrome (MS). Interest in the beneficial effects of Cannabis sativa L. has increased in recent years. Cannabinoids such as tetrahydrocannabinol (THC) and cannabidiol (CBD) have been found to be potent antioxidants. The aim of this study was to evaluate the effects of cannabis oil on liver injury and oxidative stress in insulin-resistant dyslipidemic rats fed a sucrose-rich diet (SRD). Male Wistar rats were fed the following diets for 21 days: Reference Diet (RD): standard commercial laboratory diet, Sucrose rich diet (SRD) and DRS+Cannabis oil (DRS+Ca): the oral administration of 1 mg/kg of body weight of cannabis oil daily. The cannabis oil presented a ratio of total cannabinoids THC:CBD of 1:2. We analized: a) Serum: triglycerides, cholesterol, glucose, transaminases (AST and ALT), alkaline phosphatase (AP) and non-enzymatic antioxidant capacity (FRAP). b) liver: triglyceride content, transaminases AST and ALT, AP, reactive oxygen species (ROS), substances reactive to thiobarbituric acid (TBARS), FRAP and Catalase activity. Results: In the DRS+AC group, serum triglyceride and cholesterol levels decreased significantly, reaching similar values to the RD group, without changes in glucose levels. In addition, AST, ALT, AP and TBARS levels were decreased (P