IMSATED   26825
INSTITUTO MULTIDISCIPLINARIO DE SALUD, TECNOLOGIA Y DESARROLLO
Unidad Ejecutora - UE
artículos
Título:
Actin filaments modulate electrical activity of brain microtubule protein two-dimensional sheets.
Autor/es:
GUTIERREZ BC; GUTIERREZ BC; CANTIELLO HF; CANTIELLO HF; CANTERO MR; CANTERO MR
Revista:
Cytoskeleton
Editorial:
Wiley Online Library
Referencias:
Año: 2020
ISSN:
1949-3584
Resumen:
The cytoskeleton of eukaryotic cells contains networks of actin filaments and microtubules (MTs) that are jointly implicated in various cell functions, including cell division, morphogenesis, and migration. In neurons, this synergistic activity drives both the formation of axons during development and synaptic activity in mature neurons. Both actin filaments and MTs also are highly charged polyelectrolytes that generate and conduct electrical signals. However, no information is presently available on a potential electrical crosstalk between these two cytoskeletal networks. Herein we tested the effect of actin polymerization on the electrical oscillations generated by two-dimensional sheets of bovine brain microtubule protein (2D-MT). The voltage-clamped 2D-MT sheets displayed spontaneous electrical oscillations representing a synchronous 224% change in conductance, and a fundamental frequency of 38 Hz. At 60 mV, a 4.15 nC of integrated charge transferred per second increased by 72.3% (7.15 nC) after addition of monomeric (G)-actin. This phenomenon had a 2-min lag time, and was prevented by the presence of the G-actin-binding protein DNAse I. Addition of prepolymerized F-actin, however, had a rapid onset (