PERSONAL DE APOYO
BUSTINGORRY Jose Fernando
artículos
Título:
Phenotypic plasticity in freshwater picocyanobacteria
Autor/es:
HUBER M. P. ; DIOVISALVI N.; FERRARO M. ; METZ S.; LAGOMARSINO L.; LLAMES M. E.; ROYO-LLONCH M.; BUSTINGORRY J.; ESCARAY R.; ACINAS S. G.; GASOL J. M.; UNREIN F.
Revista:
ENVIRONMENTAL MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2016
ISSN:
1462-2912
Resumen:
Picocyanobacteria can occur as single-cell (Pcy) or ascolonies (CPcy). Published evidence suggests thatsome Pcy strains have the capability to aggregateunder certain culture conditions, however this has notbeen demonstrated to occur in natural environments.We investigated whether the Pcy and CPcy belong tothe same species (i.e. phylotype), and the factors thatdetermine their morphological and genetic variabilityin a hypertrophic shallow lake dominated by picocyanobacteria.Six main different morphologies and >30phylotypes were observed. All sequences retrievedbelonged to the ?Anathece1Cyanobium? clade (Synechococcales)that are known to have the capability ofaggregation/disaggregation. The temporal variation ofpicocyanobacteria morphotype composition wasweakly correlated with the DGGE temporal pattern,and could be explained by the composition of the zooplanktonassemblage. Laboratory experimentsconfirmed that the small cladoceran Bosmina favouredthe dominance of CPcy, i.e. Cyanodictyondoubled the size of the colonies when present, mostlikely through the aggregation of single-cell picocyanobacteriainto colonies. Flow cytometry cell sortingand 16S rRNA1ITS sequencing of the Pcy and CPcycytometrically-defined populations revealed that somephylotypes could be found in both sorted populations,suggesting phenotypic plasticity in which variousSynechococcales phylotypes could be found in situeither as single-cells or as colonies.