Changes in the Antioxidant Activity of Peptides Released during the Hydrolysis of Quinoa (Chenopodium quinoa willd) Protein Concentrate

LOBO, MANUEL OSCAR; SAMMÁN, NORNMACRISTINA; RUEDA, JULIO

Proceedings

MDPI

Año: 2020 vol. 53

There is an increased interest in Andean crops as sources of nutritious compounds. Thisstudy evaluated changes in the antioxidant activity of quinoa protein hydrolysate with commercialenzymes. Aliquots at 0, 30, 60, 120 and 180 min were tested for DPPH (2,2′-diphenyl-1-picrylhydrazyl) and ABTS ((2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), antiradicalactivity. Initial DPPH inhibition rose from 9.2% ± 2.7% to 20.0% ± 4.0% (30 min) when employingalcalase and initial ABTS inhibition increased from 20.9 ± 0.2 to 105.1 ± 3.7 with ascorbic acid µg/mL(30 min). Protamex improved this to 75.7 ± 0.6 µg/mL (180 min). Alcalase and protamex are suitableenzymes for the production of rich peptides and hydrolysates as novel ingredients with antioxidantactivity.