INBIOFIV   26685
INSTITUTO DE BIOPROSPECCION Y FISIOLOGIA VEGETAL
Unidad Ejecutora - UE
artículos
Título:
Tetraglochin andina Ciald.: A medicinal plant from the Argentinean highlands with potential use in vaginal candidiasis
Autor/es:
MORENO, MARÍA ALEJANDRA; MERCADO, MARÍA INÉS; NADER-MACIAS, M.E. FATIMA; SCHMEDA-HIRSCHMANN, GUILLERMO; CÓRDOBA, SUSANA; PONESSA, GRACIELA; SAYAGO, JORGE; ISLA, MARÍA INÉS; ZAMPINI, IRIS CATIANA; ALBERTO, MARÍA ROSA; BURGOS-EDWARDS, ALBERTO
Revista:
JOURNAL OF ETHNOPHARMACOLOGY
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Año: 2018 vol. 216 p. 283 - 283
ISSN:
0378-8741
Resumen:
ETHNOPHARMACOLOGICAL RELEVANCEThe Argentinean medicinal plant Tetraglochin andina Ciald, formerly classified as T. cristatum (Britton) Rothm is used in traditional medicine by inhabitants from Argentinean northwestern highlands (Puna) to treat candidiasis and as anti-inflammatory.AIM OF THE STUDYTo assess the potential of the crude drug as an anti-Candida agent with anti-inflammatory properties. The bioactivity and phytochemical composition of a dry extract of the plant was investigated.MATERIAL AND METHODSThe pharmacognostic description of the crude drug is carried out for the first time, including macroscopic and microscopic examinations of the different organs, physicochemical and extractive values (petroleum ether-, ethanol- and water-soluble). The dry extract from T. andina was evaluated as antifungal against pathogenic Candida sp. and Saccharomyces cerevisiae isolated from vaginal infections and reference strains, by the macrodilution and microdilution assays. The normal vaginal microbiome in women is characterized by the dominance of lactic acid-producing bacteria, mainly Lactobacillus spp. The effect of T. andina extract on Lactobacillus strains was also assayed. The inhibitory effect on proinflammatory enzymes (cyclooxygenase, lipoxygenase and phospholipase A2) and antioxidant capacity was studied. The chemical profile was analyzed by HPLC-ESI-MS.RESULTSThe hydroalcoholic extract inhibited the growth of all yeasts with Minimal Inhibitory Concentration (MIC) values between 12.5 and 400 µg GAE/mL and the MIC values on Lactobacillus were higher than the MIC values against Candida isolates ( > 400 µg GAE/mL). These results indicate that the hydroalcoholic extract could be used without affecting the normal microbiota of vaginal fluid. The extract showed antioxidant activity and could modulate the inflammatory process by three pathways (sPLA2, COX-2, LOX). The plant extract contained high total phenolic levels (386.9±1.7 mg GAE/g dry extract) and flavonoid levels (260.4±2.7 mg GAE/g dry extract). Fifty phenolic compounds were identified by HPLC-ESI-MS. They were mainly hydrolysable and condensed tannins. The dry extract was chemically and biologically stable during one year at room temperature or 4 °C.CONCLUSIONSThe presence of anti-Candida and anti-inflammatory activities in Tetraglochin andina extracts give support to their traditional use for treating conditions associated with microorganism infections and inflammatory process in humans. This plant preparation could be used to design phytopharmaceutical preparations to inhibit yeast growth and moderate the inflammatory and oxidative process. (Less)
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