IC   26529
INSTITUTO DE CALCULO REBECA CHEREP DE GUBER
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Computational modeling of intracellular motor-driven transport of dynamic organelles
Autor/es:
BRUNO, LUCIANA; FERNÁNDEZ CASAFUZ, AGUSTINA BELÉN
Lugar:
São Paulo
Reunión:
Workshop; APS & ICTP-SAIFR Young Physicists Forum on Biological Physics: from Molecular to Macroscopic Scale (Bio2020); 2020
Institución organizadora:
ICTP-SAIFR
Resumen:
Intracellular organization depends on molecular motors that transport organelles and other cargoes along microtubules and actin filaments. In order to understand the behavior of molecular motors during intracellular transport several models have been presented, mainly dealing with the motion of spherical rigid organelles. It has been determined that the transport usually requires the conjoint action of multiple copies of motors and that their spatial distribution may affect the transport. For this reason, it is important to further explore the action of molecular motors when driving cargoes with different geometries and variable elasticity.In this work, we use numerical simulations to analyze the 2D motion of an elongated and flexible organelle driven by several copies of molecular motors along a microtubule in the sein of a very viscous cytoplasm. The model assumes an internal degree of freedom for the organelle that allows it to deform in a damped elastic potential. In the simulations, motors undergo bidimensional diffusion on the cargo, attach to the microtubule, step on it and detach from the track, stochastically. We also study the behavior of the organelle and motors when they encounter a second microtubule. The system is numerically integrated to obtain the organelle trajectory and deformation -i.e. length and curvature variation- during the transport, as well as the distribution of active motors along the organelle.These results are then compared with experimental observations of mitochondria during active motion in Xenopus Laevis melanophores.