Metabolic syndrome in female mouse: effect on fertility and ovarian angiogenesis.

PARERA VE; DALTON GN; COHEN DJ; SCOTTI L; PASCUALI N; OUBINA G; NEGRI MONTES L; DE SIERVI A; PARBORELL F; ABRAMOVICH D

Mar del Plata

Congreso; LXIII Reunión Annual de la Sociedad Argentina de Investigación Clínica; 2018

Sociedad Argentina de Investigación Clínica

Metabolic syndrome (MS) is a cluster of conditions that comprisesat least three of these features: overweight, hypertension, hypertriglyceridemia,hyperglycemia and low HDL. Metformin (MET) is ahypoglycemic drug used for type 2 diabetes. MET is capable of improvingfertility in these women.Objective: To evaluate oocyte quality, in vitro fertilization, folliculardevelopment, and ovarian Platelet-Derived Growth Factor B (PDGFB)in a MS female mouse model. To study ovarian effects of MET.Methods: C57BL/6 female mice, four weeks old, were fed with a highfat diet (HFD) during 17 weeks. Control animals received standarddiet (SD). A group of HFD also received MET. The estrous cycle andglycemia was evaluated. The animals were sacrificed and serumwas collected to measure cholesterol and triglycerides. One ovarywas used to obtain proteins and the other for histological studies.Another set of animals was superovulated with eCG and hCG. Animalswere sacrificed and the number of ovulated oocytes counted.By an in vitro fertilization we measured the percentage of fertilizedoocytes and calculated the percentage of these oocytes that developedto blastocyst stage.Results: HFD group showed higher weight, cholesterol and triglyceridemia.Not differences were found in the number of ovulated or fertilizedoocytes. The percentage of embryos that developed to blastocystwas lower in the HFD group. MET reversed this effect. The HFDgroup spent more time in diestrous and less time in estrous and theMET group spent more time in metaestrous and less time in estrouscompared to HFD group. Also, MET raised ovarian PDGFB. Finally,follicular dynamics was altered in HFD animals and MET improvedit, raising the percentage of corpora lutea and reducing the percentageof atretic follicles.Conclusions: MS alters follicular dynamics and oocyte quality. METadministration would have a beneficial effect on fertility in MS.