INVESTIGADORES
DE SIERVI Adriana
congresos y reuniones científicas
Título:
Could BRCA1 be a master switch for transcriptional control of genome stability?
Autor/es:
DE SIERVI A; JACOBS J; RUSTICI G; GARDNER K
Lugar:
Los Angeles, California, USA
Reunión:
Congreso; 98th AACR Annual Meeting; 2007
Institución organizadora:
American Association for Cancer Research
Resumen:
The breast cancer associated gene, BRCA1, plays multiple roles in transcription, cell cycle progression, chromatin structure and genome stability. Though many of the protein interaction partners of BRCA1 have been characterized, little is known about the direct targets of BRCA1-mediated transcriptional control. Using a combination of chromatin immuno-precipitation and microarray technology we have profiled the genome-wide interaction of BRCA1 with the proximal promoters in mammalian cells. The results of this analysis suggest that BRCA1 forms complexes at the promoter of numerous genes that are involved in the control of cell cycle progression, chromatin structure and DNA repair. Surprisingly, one of these genes is the BRCA1 gene itself. We provide data that demonstrates that BRCA1 participates in an autoregulatory loop that directly controls it own expression in response to genotoxic stress and progression through the cell cycle.