APICHELA Silvana Andrea
Effect of mating on mRNA and protein expression of beta nerve growth factor and its receptor, TrKA, in the oviduct of llama ( Lama glama )
SARI, LUCIANA M.; ZAMPINI, RENATO; ARGAÑARAZ, MARTIN E.; RATTO, MARCELO H.; APICHELA, SILVANA A.
MOLECULAR REPRODUCTION AND DEVELOPMENT
WILEY-LISS, DIV JOHN WILEY & SONS INC
Copulation produces different stimuli in the female reproductive tract in camelids, which lead to ovulation. Expression of β-NGF and its specific receptor, TrKA, was studied comparing the oviductal microenvironment of mated and non-mated llamas. β-NGF and TrKA were expressed in the llama ampulla, isthmus and utero-tubal-junction (UTJ), and they were mainly co-localized in the apical region of the oviductal mucosa. A TrKA immunosignal was also found in muscle cells and blood vessels, with the highest mark in UTJ muscle cells of copulated females. Both β-NGF and TrKA transcripts were expressed in the three oviductal segments. Relative TrKA abundance did not differ between mated and non-mated females, but relative β-NGF abundance was higher in the UTJ of copulated females (p