APICHELA Silvana Andrea
CHANGES IN MUCINS AND MATRIX METALLOPROTEASES IN THE ENDOMETRIUM OF EARLY PREGNANT ALPACAS (Vicugna pacos)
BARRAZA, DANIELA E.; ZAMPINI, RENATO; APICHELA, SILVANA A.; PACHECO, JOEL; ARGAÑARAZ, MARTIN E.
ELSEVIER GMBH, URBAN & FISCHER VERLAG
Lugar: ALEMANIA; Año: 2018 vol. 120 p. 438 - 445
During early pregnancy the uterine epithelial surface undergoes ultrastructural, biochemical and genetic changes to allow embryo implantation. In South American Camelids (SAC), this is an uncharacterized process with unique features and as a result 98% of the pregnancies develop in the left uterine horn. The present study describes the uterine horn luminal surface and the characteristics of the mucinous glycocalix in non-pregnant and early pregnant (15 days) female alpacas. In addition, the relative abundance of Mucin 1 and 16 genes (MUC1 and MUC16) wasdetermined, as well as the relative mRNA abundance of matrix metalloproteinases (MMPs) that could be involved in MUC shedding during early pregnancy. Differences between pregnant uterine horns were not observed, but noticeablechanges were detected in the uterine luminal epithelium and glycocalix, as well as presence of MUCs and MMPs in the endometrial environment in pregnant alpacas. The decrease in glycocalix staining and in the relative abundance of MUC 1 and MUC 16 transcripts would allow embryo attachment to the luminal epithelium and its subsequent implantation, as has been described in other mammals. These results suggest a crucial role of MUC1 and MUC16 and a possible role of MMPs in successful embryo implantation and survival in alpacas.